Planova 20n virus filter

The combination of these two tests maximizes the range of detectable filter defects and thus enhances the safety and reliability of virus removal processes. Please contact us and cite the number below to order. The Gold Particle Test, which is conducted by introducing colloidal gold particles matching the targeted virus sizes into the filter membrane, is performed after filter use to confirm that the fiber pore size distribution has not changed considerably during filtration.

The system allows for easy and speedy integrity testing of Planova filters, allowing for a more efficient operation process. The Leakage Test, which is an air-diffusion-based test, is conducted before and after filter use to confirm that the filter is free from pinholes or large membrane defects which may have occured during shipping or during filtration.

It can be performed either manually via visual detection of pressurized air bubbling through the hollow fibers or automatically using the Planova Leak Tester for filters with effective surface area size of 0. The table below lists the catalog number for Planova Leak Tester and its parts. Planova 75N filter is designed to remove impurities or aggregated proteins prior to final virus filtration.

The filter is available in various surface area sizes to support the use from lab to manufacturing scales. The table below lists the catalog number for Planova 75N.

Accessories to support fitting and connection of Planova filters with surface area sizes of 0. Find what you need. The Pioneer Virus Filter with Proven Reliability Since As the world's first filters developed specifically for removing viruses from biotherapeutic drug products, Planova filters have been leading the virus filtration industry.

Filter Selection Guide. Featured Articles Microscopic visualization of virus removal by dedicated filters used in biopharmaceutical processing: Impact of membrane structure and localization of captured virus particles, Adan-Kubo, J. Applications First launched in , Planova filters containing regenerated cellulose hollow fiber membranes have a long history of application in biopharmaceutical industry.

All Trends for Trends for Advanced Therapy Biomanufacturing as a Service. Flow Cytometry as Process Analytical Technology. Seven Biopharma Trends to Watch in Top 10 Spatial Biology Companies. All Rights Reserved. The objective of this study was to use confocal and scanning electron microscopy to directly evaluate the capture of virus-size nanoparticles in Planova 20N and BioEX hollow-fiber virus filters.

Confocal images of fluorescent nanoparticles were quantified using ImageJ image processing software based on the measured fluorescence intensity of the labeled nanoparticles. Structure and filtration performances of improved cuprammonium regenerated cellulose hollow fiber improved BMM hollow fiber for virus removal. Article Google Scholar. Yamaguchi, K. Electron microscopic estimation of removal of parvovirus B19 HPVB19 by nanofiltration with a novel filter membrane.

Hongo-Hirasaki, T. Effects of varying virus-spiking conditions on a virus-removal filter Planova 20N in a virus validation study of antibody solutions. Adan-Kubo, J. Microscopic visualization of virus removal by dedicated filters used in biopharmaceutical processing: Impact of membrane structure and localization of captured virus particles.

Naito, T. Single-molecule pull-out manipulation of the shaft of the rotary motor F 1 -ATPase. Cetlin, D. Use of a noninfectious surrogate to predict minute virus of mice removal during nanofiltration. Johnson, S. Characterization of non-infectious virus-like particle surrogates for viral clearance applications. Thompson, R. Precise nanometer localization analysis for individual fluorescent probes. Fernandez-Suarez, M.

Fluorescent probes for super-resolution imaging in living cells. Cell Biol. Gustafsson, M. Super-resolution light microscopy goes live. Methods 5 , — Nazem-Bokaee, H. New insights into the performance characteristics of the Planova-series hollow-fiber parvovirus filters using confocal and electron microscopy.

Leisi, R. Determination of parvovirus retention profiles in virus filter membranes using laser scanning microscopy. Masaike, T. Cooperative three-step motions in catalytic subunits of F 1 -ATPase correlate with 80 degrees and 40 degrees substep rotations.

Nishizaka, T. Chemomechanical coupling in F1-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation. Download references. The authors thank Dr. Kubo, Dr. Inoue, Mr. Watanabe and Dr. Nakane for discussions that were critical to designing the research and preparing the manuscript, Dr. Kubo, Ms. Nakayama, Dr. Fujimura and Dr. Hamamoto for their early work on human parvovirus B19, Ms.

Suzuki for the technical assistance to construct the tubing system, and Mr. Sato and Ms. You can also search for this author in PubMed Google Scholar. Correspondence to Yoshiyuki Sawamura or Takayuki Nishizaka. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material.

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Ayano, M. Direct visualization of virus removal process in hollow fiber membrane using an optical microscope. Sci Rep 11, Download citation. Received : 03 September Accepted : 23 November Published : 13 January Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. By submitting a comment you agree to abide by our Terms and Community Guidelines.

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